Human Osteopontin/OPN ELISA Kit
Validation Data Gallery
Product Information
KE00233 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Osteopontin/OPN ELISA kit is to be used to detect and quantify protein levels of endogenous Osteopontin/OPN. The assay recognizes human Osteopontin/OPN. An antibody specific for Osteopontin/OPN has been pre-coated onto the microwells. The Osteopontin/OPN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Osteopontin/OPN is added to detect the captured human Osteopontin/OPN protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Osteopontin/OPN ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.85 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6696 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 83%-117% |
| Human plasma | 94% | 77%-107% |
| Cell culture supernatants | 96% | 84%-117% |
| Urine | 92% | 77%-112% |
| Human milk | 93% | 86%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,000.9 | 30.3 | 3.0 |
| 2 | 20 | 232.2 | 10.4 | 4.5 |
| 3 | 20 | 54.5 | 2.3 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 942.4 | 38.1 | 4.0 |
| 2 | 24 | 214.7 | 11.8 | 5.5 |
| 3 | 24 | 47.6 | 3.4 | 7.1 |
Background Information
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | OPN, Osteopontin, BNSP, Bone sialoprotein 1, BSPI |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Mol Sci Proteomic Changes Induced by the Immunosuppressant Everolimus in Human Podocytes | ||
Front Immunol OPN promotes pro-inflammatory cytokine expression via ERK/JNK pathway and M1 macrophage polarization in Rosacea | ||
Biochem Biophys Res Commun Pirfenidone and nintedanib exert additive antifibrotic effects by the SPP1-AKT pathway in macrophages and fibroblasts |
