Speedy™ Human OX40L/TNFSF4 One-Step ELISA Kit
Sensitivity
0.3 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human OX40L/TNFSF4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7292 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 81% | 70%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 409.8 | 12.4 | 3.0 |
| 2 | 8 | 113.5 | 4.8 | 4.2 |
| 3 | 8 | 56.0 | 2.4 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 423.3 | 21.1 | 5.0 |
| 2 | 16 | 113.9 | 4.3 | 3.8 |
| 3 | 16 | 57.0 | 2.3 | 4.1 |
Background Information
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also named as OX40 ligand (OX40L), TXGP1 and CD252. TNFSF4 (OX40L or CD134L) is the only known ligand of TNFRSF4 (OX40 or CD134). It is a type II transmembrane protein that contains the conserved tumor necrosis factor (TNF) homology domain that enables trimerization. TNFSF4 is a co-stimulatory checkpoint protein expressed by several types of immune and non-immune cells, and was shown in the past to enhance the anti-neoplastic activity of T cells. TNFSF4 has long been known to be expressed on antigen presenting cells and to be inducible in T cells . Expression of TNFSF4 by monocytes was shown to promote T follicular helper cell polarization and pathogenesis in human lupus.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B2M, Beta-2-microglobulin, OX40L, TNFSF4, TNFSF4/CD252 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
