Human OX40/CD134 ELISA Kit
Sensitivity
11.1 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00983 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human OX40/CD134 ELISA kit is to be used to detect and quantify protein levels of endogenous human OX40/CD134. The assay recognizes human OX40/CD134. An antibody specific for human OX40/CD134 has been pre-coated onto the microwells. The human OX40/CD134 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human OX40/CD134 is added to detect the captured human OX40/CD134 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human OX40/CD134 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants,Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11.1 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7293 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 70%-126% |
| Cell culture supernatant | 106% | 99%-117% |
| Cell lysate | 95% | 85%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,495.4 | 88.9 | 5.9 |
| 2 | 8 | 388.0 | 10.4 | 2.7 |
| 3 | 8 | 208.1 | 9.7 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,540.1 | 95.2 | 6.2 |
| 2 | 16 | 387.2 | 18.3 | 4.7 |
| 3 | 16 | 214.2 | 13.4 | 6.2 |
Background Information
OX40 (CD134) is a co-stimulatory receptor belonging to the TNF receptor family. It is expressed on activated CD4+ T cells and plays a crucial role in enhancing T cell proliferation, cytokine production, and survival. OX40 signaling leads to the activation of NF-κB and the upregulation of anti-apoptotic proteins such as Bcl-xL and Bcl-2. This receptor also promotes the differentiation of naive CD4+ T cells into high IL-4-producing effectors. OX40 ligand (OX40L) is expressed on antigen-presenting cells (APCs) such as activated B cells and dendritic cells. The interaction between OX40 and OX40L is critical for T cell activation and the regulation of immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF4, ACT35, ACT35 antigen, CD134, OX40 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
