Human ORM1 ELISA Kit
Cat no : KE00137
Synonyms
AGP1, Alpha 1 acid glycoprotein 1, Alpha-1-acid glycoprotein 1, OMD 1, ORM
Validation Data Gallery
Product Information
KE00137 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ORM1 ELISA kit is to be used to detect and quantify protein levels of endogenous ORM1. The assay recognizes human ORM1. An antibody specific for ORM1 has been pre-coated onto the microwells. The ORM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ORM1 is added to detect the captured ORM1 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ORM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.37 ng/mL |
| Range | 0.625-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5004 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 89% | 77%-103% |
| Human serum | 96% | 78%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 8.40 | 0.31 | 3.7 |
| 2 | 20 | 2.40 | 0.08 | 3.3 |
| 3 | 20 | 1.30 | 0.11 | 8.5 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 8.70 | 0.58 | 6.7 |
| 2 | 24 | 2.30 | 0.14 | 6.1 |
| 3 | 24 | 1.40 | 0.14 | 10.0 |
Background Information
ORM1 (Orosomucoid 1), also named AGP1 (Alpha-1-acid glycoprotein 1) and AGP A, is an abundant plasma protein characterized by anti-inflammatory and immune-modulating properties. In human, two isoforms have been described, named ORM1 and ORM2. ORM1 is implicated in two main different functions: (1) as a lipocalin family member, it exerts a carrier activity especially for drug and lipid molecules; (2) being an acute phase protein (PMID: 23973664). ORM1 are increased in the patients with cancers, burnt injury or surgical operation (PMID: 10913518; PMID: 1646598). ORM1 could be a potential novel urinary biomarker for the early detection of chronic heart failure (PMID: 25215505).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGP1, Alpha 1 acid glycoprotein 1, Alpha-1-acid glycoprotein 1, OMD 1, ORM |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Clin Lipidol Obstructive sleep apnea syndrome attenuated high-density lipoprotein function | ||
Adv Sci (Weinh) Orosomucoid 1 Ameliorates Temporomandibular Joint Osteoarthritis by Maintaining Cartilage Homeostasis. |
