Human/Mouse NF-L ELISA Kit
Validation Data Gallery
Product Information
KE10103 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse NF-L ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse NF-L. The assay recognizes human/mouse NF-L. An antibody specific for human/mouse NF-L has been pre-coated onto the microwells. The human/mouse NF-L protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human/mouse NF-L is added to detect the captured human/mouse NF-L protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse NF-L ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cerebrospinal fluid 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4747 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 77%-109% |
| Human cerebrospinal fluid | 96% | 87%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 489.3 | 15.7 | 3.2 |
| 2 | 20 | 125.7 | 7.0 | 5.6 |
| 3 | 20 | 30.9 | 1.6 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 459.4 | 22.0 | 4.8 |
| 2 | 24 | 110.9 | 8.2 | 7.4 |
| 3 | 24 | 32.9 | 1.9 | 5.7 |
Background Information
NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 160 kDa NF-M and the 200 kDa NF-H to form neurofilaments. Neurofilaments are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homomeric 10-nm filaments. Accumulations of neurofilaments are a pathologic feature of several neurodegenerative diseases, including Amyotrophic Lateral Sclerosis (ALS), Alzheimer's disease, Parkinson's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NEFL, neurofilament, NF68, NFL |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Toxics Development of a Novel Microphysiological System for Peripheral Neurotoxicity Prediction Using Human iPSC-Derived Neurons with Morphological Deep Learning | ||
Eur J Cell Biol A physiologically relevant blood-cerebrospinal fluid barrier model to study the permeability of neurodegenerative biomarkers. |
