Human NDRG1 ELISA Kit
Sensitivity
0.19 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Cat no : KE00885
Synonyms
CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1, Protein NDRG1
Validation Data Gallery
Product Information
KE00885 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NDRG1 ELISA kit is to be used to detect and quantify protein levels of endogenous human NDRG1. The assay recognizes human NDRG1. An antibody specific for human NDRG1 has been pre-coated onto the microwells. The human NDRG1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NDRG1 is added to detect the captured human NDRG1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NDRG1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.19 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10397 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 85% | 80%-93% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.36 | 0.57 | 2.66 |
| 2 | 8 | 4.87 | 0.17 | 3.53 |
| 3 | 8 | 1.39 | 0.04 | 2.83 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 20.86 | 0.79 | 3.78 |
| 2 | 16 | 4.89 | 0.18 | 3.76 |
| 3 | 16 | 1.39 | 0.05 | 3.57 |
Background Information
NDRG1 (N-myc downstream-regulated gene 1) is a widely expressed protein implicated in diverse cellular processes, including cell differentiation, stress response, and tumor suppression. It belongs to the NDRG family, which consists of four members (NDRG1-4) sharing high sequence homology. NDRG1 is regulated by multiple stimuli, such as hypoxia, DNA damage, and heavy metals (e.g., nickel and cobalt), often acting as a stress-responsive gene. Notably, NDRG1 has been extensively studied in cancer due to its dual role as both a tumor suppressor and promoter, depending on the context. It inhibits metastasis in several cancers. Conversely, elevated NDRG1 expression is associated with poor prognosis in glioblastoma and pancreatic cancer. Beyond oncology, NDRG1 mutations cause Charcot-Marie-Tooth disease type 4D (CMT4D), a hereditary neuropathy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1, Protein NDRG1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
