Human/Mouse/Rat MAP2 ELISA Kit
Sensitivity
11.0 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Human/Mouse/Rat
Validation Data Gallery
Product Information
KE00917 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat MAP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat MAP2. The assay recognizes human/mouse/rat MAP2. An antibody specific for human/mouse/rat MAP2 has been pre-coated onto the microwells. The human/mouse/rat MAP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat MAP2 is added to detect the captured human/mouse/rat MAP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat MAP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11.0 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4133 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 109% | 102%-126% |
| Lysate | 101% | 84%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,351.2 | 30.6 | 2.3 |
| 2 | 8 | 435.0 | 20.3 | 4.7 |
| 3 | 8 | 172.1 | 5.9 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,334.1 | 42.5 | 3.2 |
| 2 | 16 | 436.6 | 16.0 | 3.7 |
| 3 | 16 | 177.2 | 11.0 | 6.2 |
Background Information
Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.Multiple high molecular weight and low molecular weight MAP2 isoforms are expressed within axons, dendrites, and cell bodies. MAP2 isoforms differ in their tissue and developmental expression pattern. In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis. In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells. MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MAP 2, MAP-2, MAP2A, MAP2B, MAP2C |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
