Recombinant Human GDF8/Myostatin protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human GDF8 protein Asn24-Ser375 (Accession# O14793) with a rabbit IgG Fc tag at the N-terminus. |
| 基因ID | 2660 |
| 蛋白编号 | O14793 |
| 预测分子量 | 66.9 kDa |
| SDS-PAGE | 13 kDa, 58-65kDa and 65-85 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively. GDF8 may have cross-reactions with GDF11.
参考文献:
1. Jespersen, J G et al. Scandinavian journal of medicine & science in sports vol. 21,2 (2011) 2. Wehling, M et al. FASEB journal : official publication of the Federation of American Societies for Experimental Biology vol. 14,1 (2000) 3. George, Isaac et al. European journal of heart failure vol. 12,5 (2010): 444-53.
