Human LRRC32 ELISA Kit
Sensitivity
0.52 ng/mL
Range
0.94-60 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00119 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LRRC32 ELISA kit is to be used to detect and quantify protein levels of endogenous LRRC32. The assay recognizes human LRRC32. An antibody specific for LRRC32 has been pre-coated onto the microwells. The LRRC32 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human LRRC32 is added to detect the captured human LRRC32 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human LRRC32 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.52 ng/mL |
| Range | 0.94-60 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2615 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 85% | 78%-107% |
| Cell culture supernatants | 98% | 88%-117% |
| Tissue lysates | 108% | 86%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 32.67 | 1.66 | 5.1 |
| 2 | 20 | 7.99 | 0.33 | 4.1 |
| 3 | 20 | 2.32 | 0.06 | 2.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 32.04 | 2.56 | 8.0 |
| 2 | 24 | 7.59 | 0.67 | 8.8 |
| 3 | 24 | 1.79 | 0.19 | 10.8 |
Background Information
LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats. LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells. It has been demonstrated as a Treg-specific activation marker. LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor. Membrane bound LRRC32 can be shed from cell surface and exists in soluble form.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | D11S833E, GARP, Garpin, LRRC32 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
