Human LIGHT/TNFSF14 ELISA Kit
Sensitivity
7.0 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00291 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LIGHT/TNFSF14 ELISA kit is to be used to detect and quantify protein levels of endogenous human LIGHT/TNFSF14. The assay recognizes human LIGHT/TNFSF14. An antibody specific for human LIGHT/TNFSF14 has been pre-coated onto the microwells. The human LIGHT/TNFSF14 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human LIGHT/TNFSF14 is added to detect the captured human LIGHT/TNFSF14 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LIGHT/TNFSF14 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant，Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8740 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 82%-105% |
| Cell culture supernatant | 97% | 73%-129% |
| Saliva | 106% | 90%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,765.0 | 56.5 | 3.2 |
| 2 | 20 | 402.0 | 10.1 | 2.5 |
| 3 | 20 | 105.6 | 4.6 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,762.8 | 58.1 | 3.3 |
| 2 | 24 | 419.9 | 11.1 | 2.6 |
| 3 | 24 | 106.1 | 5.8 | 5.5 |
Background Information
LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily. It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses. In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HVEML, CD258, HVEM L, LIGHT, LTg, TNFSF14, TR2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
