Recombinant Human LIF protein (Myc Tag, His Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
Myc Tag, His Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <1.0 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human LIF protein Ser23-Phe202 (Accession# P15018) with a Myc tag and a His tag at the C-terminus. |
| 基因ID | 3976 |
| 蛋白编号 | P15018 |
| 预测分子量 | 25.3 kDa |
| SDS-PAGE | |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF is a glycoprotein containing six putative N-glycosylation sites. The molecular weight of the unglycosylated LIF protein is 20-25 kDa, while the molecular weight of the glycosylated protein is in the range of 37-63 kDa.
参考文献:
1. Viswanadhapalli, Suryavathi et al. Genes & diseases vol. 9,4 973-980. 29 Apr. 2. Nicola, Nicos A, and Jeffrey J Babon. Cytokine & growth factor reviews vol. 26,5 (2015): 533-44. 3. Hilton, D J. Trends in biochemical sciences vol. 17,2 (1992): 72-6. 4. Fan, L et al. Journal of dental research vol. 101,1 (2022): 63-72.