Human LGALS9/Galectin-9 ELISA Kit
Cat no : KE00175
Synonyms
Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21
Validation Data Gallery
Product Information
KE00175 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LGALS9/Galectin-9 ELISA kit is to be used to detect and quantify protein levels of endogenous LGALS9/Galectin-9. The assay recognizes human LGALS9/Galectin-9. An antibody specific for LGALS9/Galectin-9 has been pre-coated onto the microwells. The LGALS9/Galectin-9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for LGALS9/Galectin-9 is added to detect the captured LGALS9/Galectin-9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LGALS9/Galectin-9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma, Human milk, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.9 pg/mL |
| Range | 31.25-2000 pg/mL, 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3965 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human milk | 94% | 74%-124% |
| Urine | 95% | 73%-108% |
| Serum | 96% | 83%-108% |
| Plasma | 96% | 78%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 105.2 | 2.9 | 2.8 |
| 2 | 20 | 258.1 | 10.9 | 4.2 |
| 3 | 20 | 887.9 | 73.7 | 8.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 113.3 | 4.6 | 4.1 |
| 2 | 24 | 291.9 | 12.9 | 4.4 |
| 3 | 24 | 1,078.3 | 64.0 | 5.9 |
Background Information
The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Hum Immunol The elevation of plasma galectin-9 levels in patients with psoriasis and its associations with inflammatory and immune checkpoint molecules in skin tissues | ||
FASEB J Novel inflammatory markers in intracerebral hemorrhage: Results from Olink proteomics analysis | ||
Front Immunol Exploring immunological alterations of B cells in peripheral immunity via single-cell RNA sequencing: insights into primary membranous nephropathy. |
