Human LDH-B ELISA Kit
Cat no : KE00287
Synonyms
lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5
Validation Data Gallery
Product Information
KE00287 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LDH-B ELISA kit is to be used to detect and quantify protein levels of endogenous LDH-B. The assay recognizes human LDH-B. An antibody specific for LDH-B has been pre-coated onto the microwells. The LDH-B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for LDH-B is added to detect the captured LDH-B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LDH-B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell Lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3945 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 113% | 86%-130% |
| Cell Lysate | 103% | 92%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 18.65 | 0.72 | 3.84 |
| 2 | 20 | 4.56 | 0.20 | 4.47 |
| 3 | 20 | 0.99 | 0.08 | 8.25 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 17.81 | 0.79 | 4.46 |
| 2 | 24 | 4.49 | 0.14 | 3.16 |
| 3 | 24 | 1.13 | 0.09 | 8.15 |
Background Information
LDH is a tetrameric enzyme that is comprised by four subunits of two types: muscle/anaerobic (LDH-A) and heart/aerobic (LDH-B). Various combinations of LDH-A subunits and LDH-B subunits (which are produced from a different gene) can form homo- or heterotetramers, and assemble into five different combinations LDH-1 (4B), LDH-2 (3B,1A), LDH-3 (2B,2A), LDH-4 (1B,3A), and LDH-5 (4A). LDH-A converts pyruvate to lactate and is expressed in the skeletal muscle, while LDH-B converts lactate to pyruvate and is expressed more in the heart muscle.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Indian J Hematol Blood Transfus Experimental Study on the Influence of Different Storage Conditions (Temperature, Duration) on Platelet Count, LDH, and Growth Factor Concentration in Platelet-Rich Plasma. |
