Human LAMR1/RPSA ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.39-25 ng/mL
Reactivity
Human
Cat no : KE00517
Synonyms
LAMR1,RPSA, RPSA, 37/67 kDa laminin receptor, 37LRP, 40S ribosomal protein SA
Validation Data Gallery
Product Information
KE00517 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LAMR1/RPSA ELISA kit is to be used to detect and quantify protein levels of endogenous human LAMR1/RPSA. The assay recognizes human LAMR1/RPSA. An antibody specific for human LAMR1/RPSA has been pre-coated onto the microwells. The human LAMR1/RPSA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human LAMR1/RPSA is added to detect the captured human LAMR1/RPSA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LAMR1/RPSA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3921 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 94% | 88%-100% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 14.69 | 1.04 | 7.10 |
| 2 | 8 | 3.23 | 0.19 | 5.72 |
| 3 | 8 | 1.60 | 0.10 | 6.02 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.64 | 1.14 | 7.76 |
| 2 | 16 | 3.36 | 0.19 | 5.78 |
| 3 | 16 | 1.69 | 0.14 | 8.59 |
Background Information
Ribosomal protein SA (RPSA), also known as laminin receptor 1 (LamR1), is a vital component of the ribosomal machinery in eukaryotic cells. It is primarily involved in the assembly and functioning of the ribosome, the cellular structure responsible for protein synthesis. It is characterized by its dual function, acting not only as a ribosomal component but also as a receptor for laminin, a key extracellular matrix protein. Ribosomal proteins, including RPSA, are known for their essential roles in ribosome assembly and protein translation, but they also exhibit ribosome-independent functions that are increasingly recognized in signaling pathways and cellular responses to external stimuli.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LAMR1,RPSA, RPSA, 37/67 kDa laminin receptor, 37LRP, 40S ribosomal protein SA |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
