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验证数据展示

  • WB analysis using 87380-1-RR

    WB analysis using 87380-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87380-1-RR (Human IgM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 87380-1-RR

    IHC staining of human tonsillitis using 87380-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87380-1-RR (Human IgM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 87380-1-RR

    IHC staining of human tonsillitis using 87380-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87380-1-RR (Human IgM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgM antibody included
  • Affinity of 87380-1-RR

    Affinity of 87380-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87380-1-RR against Human IgM were performed. The affinity constant is 0.827 nM.

Human IgM Recombinant monoclonal antibody

Human IgM Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87380-1-RR

产品说明书

CloneNo. 252244E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

IGHM, IgM, MU, VH

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87380-1-RR targets Human IgM in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6742

Product name: Recombinant Human IgM protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 105-448 aa of BC020240

Sequence: VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin heavy constant mu
别名 IGHM, IgM, MU, VH
观测分子量75 kDa
GenBank蛋白编号BC020240
基因名称 IGHM
Gene ID (NCBI) 3507
RRIDAB_3745499
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgM (~75 kDa).

实验方案

Product Specific Protocols
IHC protocol for Human IgM antibody 87380-1-RRDownload protocol
WB protocol for Human IgM antibody 87380-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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