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IgG Kappa chain Monoclonal antibody

Human IgG Kappa chain Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

4D11F7

Cat No : 67761-1-Ig

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 67761-1-Ig

    WB analysis of human plasma using 67761-1-Ig

    16 nL human plasma were subjected to SDS PAGE followed by western blot with 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67761-1-Ig targets Human IgG Kappa chain in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human
Immunogen nature protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Human IgG Kappa chain
Synonyms
Calculated Molecular Weight 155 kDa
Observed Molecular Weight~25 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918529
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the Kappa chain of human IgG,

实验方案

Product Specific Protocols
WB protocol for Human IgG Kappa chain antibody 67761-1-IgDownload protocol
IHC protocol for Human IgG Kappa chain antibody 67761-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols