Human IL-34 ELISA Kit
Validation Data Gallery
Product Information
KE00151 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL34 ELISA kit is to be used to detect and quantify protein levels of endogenous IL34. The assay recognizes human IL34. An antibody specific for IL34 has been pre-coated onto the microwells. The IL34 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL34 is added to detect the captured IL34 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-34 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 9.7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 146433 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 102% | 90%-113% |
| Human milk | 113% | 96%-130% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1056.3 | 45.3 | 4.3 |
| 2 | 20 | 239.3 | 11.8 | 4.9 |
| 3 | 20 | 56.0 | 3.8 | 6.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1022.2 | 75.4 | 7.4 |
| 2 | 24 | 234.0 | 21.5 | 9.2 |
| 3 | 24 | 53.3 | 5.2 | 9.8 |
Background Information
Interleukin 34 (IL34) is a hematopoietic cytokine that acts as a key regulator of survival, proliferation and differentiation of myeloid lineage cells including monocytes, macrophages and osteoclasts. IL34 was identified in 2008 in a comprehensive proteomic analysis as a tissue-specific ligand of CSF-1 receptor (CSF-1R). This cytokine shares numerous common features with M-CSF, especially its receptor (MCSF-R) explaining partly their functional overlap. IL34 is expressed in various tissues and it is most abundant in the spleen. Upon inflammation, other cells, such as fibroblasts and articular synovial cells, upregulate IL34 expression. Recent studies have reported that IL34 levels are elevated in the serum, synovial fluid, and synovial tissue of RA patients, and like CSF-1, IL34 is induced by TNF and IL-1beta in RA fibroblast-like synoviocytes (FLS).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C16orf77, IL 34, IL34, IL-34, interleukin 34 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Neuroimmunol Increased serum interleukin-34 levels as a novel diagnostic and prognostic biomarker in patients with acute ischemic stroke. |
