Human IL-28B ELISA Kit
Cat no : KE00014
Synonyms
Cytokine Zcyto22, IFN lambda 3, IFN lambda 4, IFNL3, IFNL4, IL 28B, IL 28C, IL-28, IL28B, IL-28B, IL28C, Interferon lambda 3, Interferon lambda 4, Interleukin 28B, Interleukin 28C, ZCYTO22
Validation Data Gallery
Product Information
KE00014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28B ELISA kit is to be used to detect and quantify protein levels of endogenous IL28B. The assay recognizes human IL28B. An antibody specific for IL28B has been pre-coated onto the microwells. The IL28B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL28B is added to detect the captured IL28B protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-28B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 282617 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plamsa | 104% | 86%-122% |
| Cell culture supernatants | 97% | 85%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 577.0 | 23.2 | 4.0 |
| 2 | 20 | 189.4 | 7.7 | 4.0 |
| 3 | 20 | 45.8 | 2.3 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 567.3 | 28.7 | 5.1 |
| 2 | 24 | 176.1 | 6.3 | 3.6 |
| 3 | 24 | 39.8 | 2.3 | 5.8 |
Background Information
IL28B, also named as IFNL3, is a cytokine distantly related to type I interferons and the IL-10 family. IL28B, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cytokine Zcyto22, IFN lambda 3, IFN lambda 4, IFNL3, IFNL4, IL 28B, IL 28C, IL-28, IL28B, IL-28B, IL28C, Interferon lambda 3, Interferon lambda 4, Interleukin 28B, Interleukin 28C, ZCYTO22 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Virol The interferon type I/III response to respiratory syncytial virus infection in airway epithelial cells can be attenuated or amplified by antiviral treatment. | ||
Med Microbiol Immunol Role of serum level and genetic variation of IL-28B in interferon responsiveness and advanced liver disease in chronic hepatitis C patients. |
