Human IL-13 ELISA Kit

Sensitivity

19.3 pg/mL

Range

62.5-4000 pg/mL

Reactivity

Human

Cat No : KE00299

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Synonyms

ALRH, BHR1, IL 13, IL13, interleukin 13, NC30, P600



Product Information

KE00299 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-13 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-13. The assay recognizes human IL-13. An antibody specific for IL-13 has been pre-coated onto the microwells. The IL-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-13 is added to detect the captured IL-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human IL-13 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant
Assay type Sandwich
Sensitivity 19.3 pg/mL
Range 62.5-4000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3596

Recovery

Sample TypeAverageRange
Human serum89%78%-98%
Cell culture supernatant100%93%-105%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120109.58.98.1
220432.324.25.6
3201,840.4168.09.1

InterAssay

Samplenmean ( pg/mL)SDCV%
124123.09.37.6
224463.321.04.5
3241,785.2101.25.7

Background Information

IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma. IL-13 has effects on immune cells that are similar to those of the closely related cytokine IL-4.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms ALRH, BHR1, IL 13, IL13, interleukin 13, NC30, P600