AuthentiKine™ Human IL-12 p40 ELISA Kit
Sensitivity
0.47 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00240
Synonyms
IL-12/IL-23 p40, IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40
Validation Data Gallery
Product Information
KE00240 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-12 p40 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-12 p40. The assay recognizes human IL-12 p40. An antibody specific for IL-12 p40 has been pre-coated onto the microwells. The IL-12 p40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-12 p40 is added to detect the captured IL-12 p40 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-12 p40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.47 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3593 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 92% | 80%-121% |
| Human urine | 95% | 85%-110% |
| Cell culture supernatants | 104% | 92%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 965.3 | 20.8 | 2.2 |
| 2 | 20 | 209.6 | 3.4 | 1.6 |
| 3 | 20 | 49.8 | 1.5 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 953.9 | 33.7 | 3.5 |
| 2 | 24 | 202.0 | 6.4 | 3.2 |
| 3 | 24 | 48.7 | 2.0 | 4.0 |
Background Information
Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. IL-12 p40 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL-12 p40 was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL-12 p40 has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL-12/IL-23 p40, IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
