Human IFN-gamma ELISA Kit
Sensitivity
1.1 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00781 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous human IFN-gamma. The assay recognizes human IFN-gamma. An antibody specific for human IFN-gamma has been pre-coated onto the microwells. The human IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IFN-gamma is added to detect the captured human IFN-gamma protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IFN-gamma ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3458 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 91%-110% |
| Cell culture supernatant | 101% | 93%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 242.2 | 10.6 | 4.4 |
| 2 | 8 | 61.5 | 3.1 | 5.1 |
| 3 | 8 | 33.5 | 1.3 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 246.4 | 16.3 | 6.6 |
| 2 | 16 | 63.9 | 3.9 | 6.0 |
| 3 | 16 | 33.8 | 1.5 | 4.3 |
Background Information
Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays a crucial role in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IFNG, IFG, IFN gamma, IFN γ, IFNγ |