AuthentiKine™ Human HGF ELISA Kit
Cat no : KE00168
Synonyms
F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF
Validation Data Gallery
Product Information
KE00168 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HGF ELISA kit is to be used to detect and quantify protein levels of endogenous HGF. The assay recognizes human HGF. An antibody specific for HGF has been pre-coated onto the microwells. The HGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, the horseradish peroxidase (HRP)-conjugated detection antibody specific for HGF is added to detect the captured HGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human HGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.26 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3082 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 91% | 73%-112% |
| Human plasma | 91% | 73%-112% |
| Cell culture supernatants | 117% | 93%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.7 | 0.30 | 6.4 |
| 2 | 20 | 9.3 | 0.65 | 7.0 |
| 3 | 20 | 18.3 | 0.92 | 5.0 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.1 | 0.42 | 8.4 |
| 2 | 24 | 10.5 | 0.96 | 9.2 |
| 3 | 24 | 20.9 | 19.3 | 9.2 |
Background Information
Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa alpha-subunit (containing four kringle domains) and the 34 kDa beta-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Drug Des Devel Ther Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis | ||
QJM Predicting survival and immune microenvironment in colorectal cancer: a STAT signaling-related signature. | ||
Sci Rep Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels | ||
Int J Mol Sci Transplantation of Chemical Compound-Induced Cells from Human Fibroblasts Improves Locomotor Recovery in a Spinal Cord Injury Rat Model | ||
Cytotherapy Mesenchymal stem cell-derived protein extract induces periodontal regeneration | ||
Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma |
