Speedy™ Human HER2/ErbB2 One-Step ELISA Kit
Sensitivity
7.8 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human HER2/ErbB2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Human milk, Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2064 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 102% | 82%-117% |
| Cell culture supernatant | 110% | 103%-117% |
| Urine | 93% | 76%-118% |
| Human milk | 111% | 105%-117% |
| Lysate | 101% | 94%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,920.4 | 25.6 | 1.3 |
| 2 | 8 | 559.1 | 9.4 | 1.7 |
| 3 | 8 | 331.0 | 28.5 | 8.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,912.2 | 35.7 | 1.9 |
| 2 | 16 | 558.8 | 9.6 | 1.7 |
| 3 | 16 | 327.0 | 27.8 | 8.5 |
Background Information
The human epidermal growth factor receptor (HER) family of receptors plays a central role in the pathogenesis of several human cancers. HER2 (human epidermal growth factor receptor 2), also known as ErbB2 and Neu, is 1 of the 4 membrane receptor tyrosine kinases (RTKs) and when activated affects cell proliferation and survival. HER2 is expressed in many tissues and its major role in these tissues is to facilitate excessive/uncontrolled cell growth and tumorigenesis. Additionally, HER2 mutations have also been reported in common cancers such as pulmonary, colorectal, and breast cancers, indicating a potential for HER2-directed therapies in these cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ERBB2, CD340, HER 2, HER 2/neu, HER2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
