Human Granzyme B ELISA Kit

Sensitivity

2.6 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat No : KE00121

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Synonyms

CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, GZMB, HLP, Homo sapiens (Human), SECT



Product Information

KE00121 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Granzyme B ELISA kit is to be used to detect and quantify protein levels of endogenous Granzyme B. The assay recognizes human Granzyme B. An antibody specific for Granzyme B has been pre-coated onto the microwells. The Granzyme B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Granzyme B is added to detect the captured Granzyme B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human Granzyme B ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 2.6 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3002

Recovery

Sample TypeAverageRange
Human plasma85%77%-95%
Cell culture supernatants91%77%-99%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12026.61.03.8
220112.05.24.6
320465.731.76.8

InterAssay

Samplenmean ( pg/mL)SDCV%
12427.42.79.7
224119.47.26.0
324499.827.75.5

Background Information

Granzyme B (GZMB) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease Granzyme B (GZMB) can promote apoptosis through direct processing and activation of members of the caspase family. Granzyme B also functions as processing protease of cytokines, including interleukin (IL)-18 and IL-1a, and extracellular matrix proteins, including fibronectin and various proteoglycans. Plasma Granzyme B concentrations were significantly higher in patients with Atopic dermatitis (AD) and psoriasis than in healthy controls, which indicated that Granzyme B may play an important role in the induction of intractable itch and dermatitis in AD.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, GZMB, HLP, Homo sapiens (Human), SECT

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Life Sci

IFNβ-induced exosomal linc-EPHA6-1 promotes cytotoxicity of NK cells by acting as a ceRNA for hsa-miR-4485-5p to up-regulate NKp46 expression.

Authors - Shuang Li
humancell culture supernatants

Nanoscale

Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic Fe(3)O(4) nanoparticles for magnetic targeting immunotherapy of solid tumors

Authors - Songbo Zhao