Human/Cynomolgus Monkey Galectin 3 ELISA Kit
Cat no : KE00311
Synonyms
Galectin-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, CBP35
Validation Data Gallery
Product Information
KE00311 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/cynomolgus monkey Galectin 3 ELISA kit is to be used to detect and quantify protein levels of endogenous human/cynomolgus monkey Galectin 3. The assay recognizes human/cynomolgus monkey Galectin 3. An antibody specific for human/cynomolgus monkey Galectin 3 has been pre-coated onto the microwells. The human/cynomolgus monkey Galectin 3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/cynomolgus monkey Galectin 3 is added to detect the captured human/cynomolgus monkey Galectin 3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Cynomolgus Monkey Galectin 3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cynomolgus monkey serum, Cynomolgus monkey plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3958 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 85%-106% |
| Cynomolgus monkey serum | 97% | 88%-102% |
| Human cell culture supernatant | 106% | 93%-121% |
| Cynomolgus monkey cell culture supernatant | 97% | 92%-100% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3.91 | 0.07 | 1.87 |
| 2 | 20 | 0.90 | 0.02 | 2.22 |
| 3 | 20 | 0.23 | 0.01 | 3.61 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3.73 | 0.13 | 3.38 |
| 2 | 24 | 0.88 | 0.03 | 2.98 |
| 3 | 24 | 0.23 | 0.01 | 4.33 |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides. Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers. Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space. Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis. Elevated serum galectin-3 levels have been reported in patients with breast, gastrointestinal, lung, or ovarian cancer, melanoma, and non-Hodgkin's lymphoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Galectin-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, CBP35 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Rep Plasma levels of soluble PD-1, TIM-3, LAG-3 and galectin-3 and the degree of liver fibrosis in CHC and the impact of successful antiviral treatment on their levels | ||
Int J Mol Sci Role of Endogenous Galectin-3 on Cell Biology of Immortalized Retinal Pigment Epithelial Cells In Vitro. | ||
Cell Commun Signal Galectin-3 in endometrial small extracellular vesicles promotes cytotrophoblast cell fusion during the implantation phase. |
