Human GRP78/BIP ELISA Kit
Cat no : KE00333
Synonyms
BIP, GRP78, HSPA5, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein
Validation Data Gallery
Product Information
KE00333 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GRP78/BIP ELISA kit is to be used to detect and quantify protein levels of endogenous human GRP78/BIP . The assay recognizes human GRP78/BIP. An antibody specific for human GRP78/BIP has been pre-coated onto the microwells. The human GRP78/BIP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human GRP78/BIP is added to detect the captured human GRP78/BIP protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GRP78/BIP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3309 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 71%-98% |
| Cell lysate | 99% | 85%-116% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.97 | 0.23 | 4.62 |
| 2 | 20 | 1.22 | 0.02 | 1.80 |
| 3 | 20 | 0.30 | 0.01 | 3.17 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.47 | 0.28 | 6.30 |
| 2 | 24 | 1.15 | 0.06 | 5.47 |
| 3 | 24 | 0.30 | 0.01 | 4.74 |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER) (PMID: 2294010; PMID: 23769672). It is a constitutively expressed resident protein of the ER in all eukaryotic cells (PMID: 23990668). It has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth (PMID: 26045166).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BIP, GRP78, HSPA5, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Pathogens Zinc Acetate Inhibits Hepatitis A Virus Replication: Possible Treatment for Patients with Type A Acute-on-Chronic Liver Failure. |
