Human GPR56 ELISA Kit
Sensitivity
0.03 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00860
Synonyms
ADGRG1, ADGRG1 CT, ADGRG1 C-terminal fragment, ADGRG1 NT, ADGRG1 N-terminal fragment
Validation Data Gallery
Product Information
KE00860 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GPR56 ELISA kit is to be used to detect and quantify protein levels of endogenous human GPR56. The assay recognizes human GPR56. An antibody specific for human GPR56 has been pre-coated onto the microwells. The human GPR56 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GPR56 is added to detect the captured human GPR56 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GPR56 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9289 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 82%-102% |
| Cell lysate | 96% | 89%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.57 | 0.14 | 2.52 |
| 2 | 8 | 1.28 | 0.04 | 2.92 |
| 3 | 8 | 0.67 | 0.03 | 4.88 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.24 | 0.37 | 7.09 |
| 2 | 16 | 1.23 | 0.07 | 5.50 |
| 3 | 16 | 0.65 | 0.03 | 5.01 |
Background Information
GPR56 (G protein-coupled receptor 56) is an adhesion G protein-coupled receptor (aGPCR) that belongs to the G protein-coupled receptor family. It regulates various cellular functions through interactions between its N-terminal GAIN domain and exogenous ligands. GPR56 plays a critical role in nervous system development, particularly in the development and myelination of oligodendrocytes. Additionally, it is involved in interactions with the extracellular matrix, binding to molecules such as collagen III and tissue transglutaminase (TG2) to regulate cell adhesion and migration. GPR56 is abnormally expressed in a variety of diseases, including liver fibrosis, depression, and multiple tumors. In liver fibrosis, GPR56 alleviates hepatocyte pyroptosis by inhibiting the NF-κB/NLRP3 pathway, thereby blocking the activation of hepatic stellate cells. In the field of oncology, GPR56 is considered a potential tumor biomarker and therapeutic target.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADGRG1, ADGRG1 CT, ADGRG1 C-terminal fragment, ADGRG1 NT, ADGRG1 N-terminal fragment |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
