Human GOLM1 ELISA Kit
Cat no : KE00111
Synonyms
bA379P1.3, C9orf155, FLJ22634, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, PSEC0257, UNQ686/PRO1326
Validation Data Gallery
Product Information
KE00111 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GOLM1 ELISA kit is to be used to detect and quantify protein levels of endogenous GOLM1. The assay recognizes human GOLM1. An antibody specific for GOLM1 has been pre-coated onto the microwells. The GOLM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for GOLM1 is added to detect the captured GOLM1 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GOLM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156 - 10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51280 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 102% | 78%-118% |
| Human plasma | 95% | 77%-114% |
| Human serum | 104% | 86%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.04 | 0.18 | 4.5 |
| 2 | 20 | 1.00 | 0.04 | 3.6 |
| 3 | 20 | 0.25 | 0.01 | 2.5 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3.91 | 0.19 | 4.8 |
| 2 | 24 | 0.96 | 0.04 | 3.8 |
| 3 | 24 | 0.23 | 0.01 | 2.7 |
Background Information
GOLM1 (also known as GP73 or GOLPH2) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLM1 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLM1 could be a promising serum marker for hepatocellular carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | bA379P1.3, C9orf155, FLJ22634, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, PSEC0257, UNQ686/PRO1326 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Open Forum Infect Dis Proteomic Analysis of Serum and Cerebrospinal Fluid in Children with Encephalopathy Associated with Human Betaherpesvirus 6B. |
