Human GNLY/Granulysin ELISA Kit
Sensitivity
0.6 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Cat no : KE00200
Synonyms
519, D2S69E, GNLY, granulysin, LAG 2, LAG2, Lymphokine LAG 2, NKG5, Protein NKG5, T cell activation protein 519, TLA519
Validation Data Gallery
Product Information
KE00200 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GNLY/Granulysin ELISA kit is to be used to detect and quantify protein levels of endogenous GNLY/Granulysin. The assay recognizes human GNLY/Granulysin. An antibody specific for GNLY/Granulysin has been pre-coated onto the microwells. The GNLY/Granulysin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GNLY/Granulysin is added to detect the captured GNLY/Granulysin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GNLY/Granulysin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10578 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 80%-111% |
| Cell culture supernatants | 82% | 75%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 259.2 | 9.5 | 3.7 |
| 2 | 20 | 66.1 | 1.9 | 2.9 |
| 3 | 20 | 14.8 | 0.5 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 262.6 | 13.7 | 5.2 |
| 2 | 24 | 60.8 | 3.9 | 6.4 |
| 3 | 24 | 13.2 | 0.7 | 5.5 |
Background Information
Granulysin (GNLY) is a member of the saposin-like protein (SAPLIP) family that is a cytolytic and proinflammatory molecule expressed by activated human cytotoxic T cells and NK cells, exhibiting lytic activity against a variety of microorganisms and tumors. Granulysin is produced as an intact 15-kDa form, and portions are then cleaved at the amino and carboxy termini to produce a 9-kDa form. Granulysin has broad clinical relevance to a wide variety of diseases, including infections, cancer, transplantation, skin afflictions and reproductive complications. Elevation of Granulysin expression and levels in tissue and serum has been reported in infections, autoimmune diseases, transplant rejection. Granulysin expression has been widely correlated with good outcomes in a variety of cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 519, D2S69E, GNLY, granulysin, LAG 2, LAG2, Lymphokine LAG 2, NKG5, Protein NKG5, T cell activation protein 519, TLA519 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
