Human GBP2 ELISA Kit
Sensitivity
3.9 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00736
Synonyms
EC:3.6.5.-, GBP 2, GBP-2, GTP-binding protein 2, Guanine nucleotide-binding protein 2
Validation Data Gallery
Product Information
KE00736 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GBP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human GBP2. The assay recognizes human GBP2. An antibody specific for human GBP2 has been pre-coated onto the microwells. The human GBP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GBP2 is added to detect the captured human GBP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GBP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.9 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2634 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 89%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,611.1 | 118.6 | 4.5 |
| 2 | 8 | 600.5 | 16.3 | 2.7 |
| 3 | 8 | 307.0 | 31.7 | 10.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,595.6 | 100.3 | 3.9 |
| 2 | 16 | 602.1 | 17.9 | 3.0 |
| 3 | 16 | 307.0 | 30.6 | 10.0 |
Background Information
Guanylate binding protein 2 (GBP2) is a member of the guanine binding protein (GBP) family that can bind to guanine nucleotides (GMP, GDP, and GTP). GBP2 is used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. GBP2 involved in various immune responses and plays a role in the host defense against pathogens. Additionally, GBP2 has been identified as a prognostic biomarker in certain cancers, including breast cancer and clear cell renal cell carcinoma. GBP2 also participates in the regulation of immune responses through pathways like STAT1 signaling.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.6.5.-, GBP 2, GBP-2, GTP-binding protein 2, Guanine nucleotide-binding protein 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
