Human GATA3 ELISA Kit
Sensitivity
37.9 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00972
Synonyms
GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
Validation Data Gallery
Product Information
KE00972 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GATA3 ELISA kit is to be used to detect and quantify protein levels of endogenous human GATA3. The assay recognizes human GATA3. An antibody specific for human GATA3 has been pre-coated onto the microwells. The human GATA3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GATA3 is added to detect the captured human GATA3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GATA3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 37.9 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2625 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 89% | 88%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,812.7 | 77.9 | 2.8 |
| 2 | 8 | 745.3 | 11.3 | 1.5 |
| 3 | 8 | 340.4 | 16.9 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,584.0 | 247.5 | 9.6 |
| 2 | 16 | 675.1 | 73.6 | 10.9 |
| 3 | 16 | 344.9 | 15.9 | 4.6 |
Background Information
GATA3 is a member of a family of transcriptional regulators with the capacity to function in determination of cell identity. It is known for its ability to bind to the DNA sequence "GATA", which enables its key role in critical processes in multiple tissues and organs, including the immune system, endocrine system, and nervous system. GATA3 also modulates cell differentiation, proliferation, and apoptosis by controlling gene expression. Its expression is associated with cell type specification in the immune system, where its action is integral to the differentiation of multiple cell types. Dysregulation of GATA3 is implicated in several diseases, such as breast cancer, hematological malignancies like T-cell lymphomas, autoimmune diseases, and neurodegenerative disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
