Human GABPA ELISA Kit
Sensitivity
5.1 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00704
Synonyms
E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain, GABP subunit alpha
Validation Data Gallery
Product Information
KE00704 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GABPA ELISA kit is to be used to detect and quantify protein levels of endogenous human GABPA. The assay recognizes human GABPA. An antibody specific for human GABPA has been pre-coated onto the microwells. The human GABPA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GABPA is added to detect the captured human GABPA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GABPA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 5.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2551 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 95% | 87%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,327.0 | 227.5 | 9.8 |
| 2 | 8 | 585.6 | 49.3 | 8.4 |
| 3 | 8 | 254.6 | 25.2 | 9.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,302.6 | 188.5 | 8.2 |
| 2 | 16 | 562.1 | 53.7 | 9.6 |
| 3 | 16 | 265.1 | 25.6 | 9.6 |
Background Information
GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. GABPA plays an important role in regulating genes involved in various biological processes such as embryonic development, cell differentiation, cell cycle, and mitochondrial biogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain, GABP subunit alpha |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
