Human Follistatin ELISA Kit
Sensitivity
15.8 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00250 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human Follistatin ELISA kit is to be used to detect and quantify protein levels of endogenous human Follistatin. The assay recognizes human Follistatin. An antibody specific for human Follistatin has been pre-coated onto the microwells. The human Follistatin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Follistatin is added to detect the captured human Follistatin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Follistatin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 15.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10468 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 86% | 73%-99% |
| Cell culture supernatants | 114% | 107%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 974.8 | 32.9 | 3.4 |
| 2 | 20 | 216.3 | 15.6 | 7.2 |
| 3 | 20 | 81.6 | 4.2 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 942.1 | 44.5 | 4.7 |
| 2 | 24 | 235.4 | 17.8 | 7.6 |
| 3 | 24 | 82.9 | 5.5 | 6.7 |
Background Information
Follistatin is a secreted glycoprotein, which is a member of the TGF-β superfamily. It was first discovered in 1987 in ovarian follicular fluid but later found to be expressed in a number of different tissues including the pituitary gland, placenta, ovary, testis, and skeletal muscle. Follistatin antagonizes the members of TGF-βsuperfamily, including activin A, growth differentiation factor 11, and myostatin. Follistatin is essential for the formation and growth of muscle fibers and is involved in the development of muscle fiber hypertrophy. Follistatin is also linked to metabolic diseases, with elevated plasma levels in patients with type 2 diabetes (T2D).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Activin binding protein, follistatin, FS, FST |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
