Human Fetuin-A ELISA Kit
Cat no : KE00097
Synonyms
A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, HSGA
Validation Data Gallery
Product Information
KE00097 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Fetuin A ELISA kit is to be used to detect and quantify protein levels of endogenous Fetuin A. The assay recognizes human Fetuin A. An antibody specific for Fetuin A has been pre-coated onto the microwells. The Fetuin A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Fetuin A is added to detect the captured Fetuin A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Fetuin-A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 ng/mL |
| Range | 0.313 - 20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 197 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 104% | 84%-125% |
| Cell culture supernatants | 106% | 81%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1.6 | 0.05 | 3.2 |
| 2 | 20 | 4.6 | 0.12 | 2.5 |
| 3 | 20 | 12.6 | 0.76 | 6.1 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1.5 | 0.04 | 2.8 |
| 2 | 24 | 4.4 | 0.23 | 5.2 |
| 3 | 24 | 12.9 | 0.48 | 3.8 |
Background Information
Fetuin A, also named as Alpha2-HS glycoprotein (AHSG), is a member of cystatin superfamily of protease inhibitors. It is a highly expressed glycoprotein in various fetal tissues whereas it is mainly expressed by the liver in adults. Fetuin A, an extracellular inhibitor of transforming growth factor β, is a profibrogenic stimulus in liver disease. Circulating fetuin A may be a beneficial serum biomarker in the detection of liver and vascular fibrosis progression in patients with non-alcoholic fatty liver disease. It is also involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuin A exerts its effects on the cardiovascular system by two different mechanisms. On the one hand it inhibits insulin signaling and induces insulin resistance contributing to the onset of atherosclerosis and on the other hand it inhibits calcium deposition and protects from vascular calcification.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, HSGA |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Am Heart Assoc Reduced Fetuin-A Levels Are Associated With Exercise Intolerance and Predict the Risk of Adverse Outcomes in Patients With Heart Failure: The Role of Cardiac-Hepatic-Peripheral Interaction | ||
Clin Res Cardiol Decreases in hepatokine Fetuin-A levels are associated with hepatic hypoperfusion and predict cardiac outcomes in patients with heart failure. | ||
J Clin Med Serum Calcification Propensity T50 Associates with Disease Severity in Patients with Pseudoxanthoma Elasticum. | ||
Sci Rep Large-scale proteomic analysis of aqueous humor from patients with primary open angle and exfoliation glaucoma. | ||
Cancer Sci Tumor-derived exosomal proteins as diagnostic biomarkers in non-small cell lung cancer. |
