Human Fas Ligand/CD178 ELISA Kit
Sensitivity
2.2 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Cat no : KE00245
Synonyms
Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 L, CD95 ligand, CD95L, Fas antigen ligand, Fas ligand, FASL, FASLG, TNFSF6
Validation Data Gallery
Product Information
KE00245 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Fas Ligand/CD178 ELISA kit is to be used to detect and quantify protein levels of endogenous human Fas Ligand/CD178. The assay recognizes human Fas Ligand/CD178. An antibody specific for human Fas Ligand/CD178 has been pre-coated onto the microwells. The human Fas Ligand/CD178 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Fas Ligand/CD178 is added to detect the captured human Fas Ligand/CD178 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Fas Ligand/CD178 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatants, Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 356 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 72%-113% |
| Cell lysates | 90% | 74%-102% |
| Cell cuture supernatants | 93% | 86%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 198.3 | 5.6 | 2.8 |
| 2 | 20 | 46.4 | 1.7 | 3.7 |
| 3 | 20 | 12.0 | 0.3 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 205.9 | 3.8 | 1.8 |
| 2 | 24 | 48.6 | 3.2 | 6.5 |
| 3 | 24 | 11.5 | 0.4 | 3.9 |
Background Information
CD178, also known as Fas ligand, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. It is expressed on NK cells, cytotoxic T lymphocytes and activated CD4+ Th1 cells. CD178 transduces apoptotic signal into cells by binding to FAS/CD95. It is involved as a death factor in the regulation of activation-induced cell death, establishment of immune privilege and tumor cell survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 L, CD95 ligand, CD95L, Fas antigen ligand, Fas ligand, FASL, FASLG, TNFSF6 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
