Speedy™ Human Fas/CD95 One-Step ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.234-15 ng/mL
Reactivity
Human
Cat no : SE50065
Synonyms
FAS, ALPS1A, APO-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Fas/CD95 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.234-15 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 355 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 97% | 92%-103% |
| Cell culture supernatant | 118% | 108%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6.12 | 0.35 | 5.72 |
| 2 | 8 | 1.61 | 0.03 | 1.86 |
| 3 | 8 | 0.86 | 0.04 | 4.65 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 6.19 | 0.37 | 5.98 |
| 2 | 16 | 1.63 | 0.07 | 4.29 |
| 3 | 16 | 0.86 | 0.05 | 5.81 |
Background Information
Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FAS, ALPS1A, APO-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
