Human FABP2 ELISA Kit
Validation Data Gallery
Product Information
KE00263 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FABP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human FABP2. An antibody specific for FABP2 has been pre-coated onto the microwells. The FABP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for FABP2 is added to detect the captured FABP2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FABP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.8 pg/mL |
| Range | 156.3-10000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2169 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 105% | 90%-123% |
| Cell Lysate | 100% | 89%-116% |
| Cell culture supernant | 100% | 83%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4,105.7 | 213.2 | 5.2 |
| 2 | 20 | 990.7 | 27.3 | 2.8 |
| 3 | 20 | 252.6 | 8.9 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4,412.0 | 271.2 | 6.1 |
| 2 | 24 | 1022.1 | 61.0 | 5.9 |
| 3 | 24 | 251.9 | 8.7 | 3.5 |
Background Information
FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FABP2, FABPI, Fatty acid binding protein 2, I FABP |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Phytomedicine Qingshu Yiqi decoction ameliorates exertional heat stroke-induced intestinal barrier injury via NF-κB/MLC pathway and gut microbiota | ||
Front Cell Dev Biol Superior thermotolerance in young versus adult rats undergoing heat stroke is associated with age-related differences in intestinal barrier integrity and heat shock protein responses. | ||
Sleep Med Acute sleep deprivation reduces thermotolerance and exacerbates heat stroke via disruption of intestinal barrier integrity in rats. |
