β-Estradiol ELISA Kit
Sensitivity
0.3 pg/mL
Range
46.88-3000 pg/mL
Validation Data Gallery
Product Information
KE30010 is a vitro competitive binding enzyme-linked immunosorbent assay(Competitive ELISA). The β-Estradiol ELISA kit is to be used to detect and quantify protein levels of vitro human β-Estradiol. The assay recognizes human β-Estradiol. A 96-well plate has been precoated with β-Estradiol antigen. Samples and the antibody-Bio conjugate are added to the wells, where β-Estradiol in the sample competes with the β-Estradiol antigen immobilized on the microplate for binding with the antibody-Bio. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of β-Estradioll in the sample and the intensity is measured at 450 nm with the correction wavelength set at 630 nm.
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 84%-128% |
| Urine | 99% | 93%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,550.9 | 68.9 | 4.4 |
| 2 | 8 | 394.1 | 34.4 | 8.7 |
| 3 | 8 | 191.2 | 16.0 | 8.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,539.4 | 72.7 | 4.7 |
| 2 | 16 | 391.1 | 36.5 | 9.3 |
| 3 | 16 | 176.6 | 24.9 | 14.1 |
Background Information
β-Estradiol is the primary and most potent estrogen sex hormone in the human body, particularly in non-pregnant females of reproductive age. It is a steroid hormone essential for the development and regulation of the female reproductive system, secondary sexual characteristics, and numerous other physiological processes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 17β-Estradiol |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
