Human Endostatin ELISA Kit
Cat no : KE00259
Synonyms
COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, endostatin, FLJ27325, FLJ34914, KNO, KNO1
Validation Data Gallery
Product Information
KE00259 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Endostatin ELISA kit is to be used to detect and quantify protein levels of endogenous human Endostatin. The assay recognizes human Endostatin. An antibody specific for human Endostatin has been pre-coated onto the microwells. The human Endostatin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Endostatin is added to detect the captured human Endostatin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Endostatin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.3 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 80781 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 109% | 96%-128% |
| Cell culture supernatants | 90% | 78%-103% |
| Saliva | 89% | 76%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 947.4 | 23.9 | 2.5 |
| 2 | 20 | 481.2 | 16.3 | 3.4 |
| 3 | 20 | 109.0 | 5.4 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 934.5 | 14.2 | 1.5 |
| 2 | 24 | 488.3 | 11.4 | 2.3 |
| 3 | 24 | 117.6 | 6.9 | 5.9 |
Background Information
Endostatin, a 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain (COL18A1), is an endogenously produced inhibitor of angiogenesis. Many different proteases are able to cleave the NC1 domain of collagen alpha-1(XVIII) chain to produce endostatin including cathepsins L, B, and K, elastase. Endostatin inhibits proliferation and migration of endothelial cells, angiogenesis, and tumor growth. It may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, endostatin, FLJ27325, FLJ34914, KNO, KNO1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion |
