Human NSE/ENO2 ELISA Kit
Cat no : KE00050
Synonyms
ENO2, NSE, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2
Validation Data Gallery
Product Information
KE00050 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NSE/ENO2 ELISA kit is to be used to detect and quantify protein levels of endogenous human NSE/ENO2. The assay recognizes human NSE/ENO2. An antibody specific for human NSE/ENO2 has been pre-coated onto the microwells. The human NSE/ENO2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NSE/ENO2 is added to detect the captured human NSE/ENO2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NSE/ENO2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2026 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 71%-118% |
| Cell culture supernatants | 90% | 70%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2.3 | 0.1 | 6.0 |
| 2 | 20 | 7.6 | 0.6 | 7.8 |
| 3 | 20 | 16.1 | 0.9 | 5.5 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2.3 | 0.1 | 5.7 |
| 2 | 24 | 7.4 | 0.4 | 5.6 |
| 3 | 24 | 12.9 | 0.8 | 5.8 |
Background Information
ENO2, also named as Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), particularly isoform neuron-specific enolase (NSE), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors. ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ENO2, NSE, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun A tissue-bioengineering strategy for modeling rare human kidney diseases in vivo. | ||
Medicine (Baltimore) Plasma S100β and neuron-specific enolase, but not neuroglobin, are associated with early cognitive dysfunction after total arch replacement surgery: A pilot study. | ||
J Mol Histol Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury. | ||
Front Aging Neurosci Transcutaneous electrical acupoint stimulation for reducing cognitive dysfunction in lumbar spine surgery: A randomized, controlled trail | ||
Ann Surg MiR-7-5p as a Diagnostic and Monitoring Biomarker for Neuroendocrine Tumors: Insights from the SKY-NET Multicenter Study | ||
Drug Alcohol Depend Peripheral inflammation and PSD-95 levels are associated with structural brain changes in individuals with nicotine dependence using e-cigarettes. |
