Recombinant Human Elastin protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human Elastin protein Gly27-Lys786 (Accession# P15502-3) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 2006 |
| 蛋白编号 | P15502-3 |
| 预测分子量 | 91.3 kDa |
| SDS-PAGE | 90-110 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Elastic fibers are an abundant and integral part of many extracellular matrices, in which they provide the elastic properties to tissues such as arterial, lung, and skin. Elastic fibers are consisting of an elastin core surrounded by a mantle of fibrillin-rich microfibrils. Elastin is an extremely durable, insoluble biopolymer formed through the lysine-mediated crosslinking of its soluble precursor tropoelastin, which is an approximately 60-70 kDa protein. Deletions and mutations in the elastin gene (ELN) are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa.
参考文献:
1. Kielty, Cay M et al. Journal of cell science vol. 115,Pt 14 (2002): 2817-28. 2. Mithieux, Suzanne M, and Anthony S Weiss. Advances in protein chemistry vol. 70 (2005): 437-61. 3. Heinz, Andrea. Critical reviews in biochemistry and molecular biology vol. 55,3 (2020): 252-273.
