Human EGFR ELISA Kit
Cat no : KE00142
Synonyms
EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1
Validation Data Gallery
Product Information
KE00142 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The EGFR ELISA kit is to be used to detect and quantify protein levels of endogenous EGFR. The assay recognizes human EGFR. An antibody specific for EGFR has been pre-coated onto the microwells. The EGFR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for EGFR is added to detect the captured EGFR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human EGFR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1956 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 74%-111% |
| Human milk | 102% | 91%-113% |
| Cell lysates | 97% | 81%-114% |
| Cell culture supernatants | 89% | 76%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,265.9 | 19.6 | 1.6 |
| 2 | 20 | 322.7 | 9.5 | 2.9 |
| 3 | 20 | 79.9 | 1.7 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,249.3 | 94.5 | 7.6 |
| 2 | 24 | 313.6 | 20.2 | 6.5 |
| 3 | 24 | 76.2 | 6.6 | 8.6 |
Background Information
The epidermal growth factor receptor (EGFR) belongs to the ErbB family of receptor tyrosine kinases (RTKs) and exerts critical functions in epithelial cell physiology. EGFR is a receptor of tyrosine kinase (RTK). It is consisted of a C-terminus intracellular region that possesses the kinase activity, and an N-terminus extracellular ligand-binding site, a hydrophobic transmembrane domain. EGFR is frequently mutated and/or overexpressed in different types of human cancers and is the target of multiple cancer therapies currently adopted in the clinical practice. Mutations in this gene are associated with lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Biol Macromol Cetuximab-based PROteolysis targeting chimera for effectual downregulation of NSCLC with varied EGFR mutations | ||
J Control Release Targeted nanoliposomes of oncogenic protein degraders: Significant inhibition of tumor in lung-cancer bearing mice |
