Human EGF ELISA Kit
Validation Data Gallery
Product Information
KE00138 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The EGF ELISA kit is to be used to detect and quantify protein levels of endogenous EGF. The assay recognizes human EGF. An antibody specific for EGF has been pre-coated onto the microwells. The EGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for EGF is added to detect the captured EGF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human EGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 26.9 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1950 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 88% | 71%-110% |
| Human serum | 92% | 71%-112% |
| Cell culture supernatants | 101% | 80%-123% |
| Saliva | 102% | 79%-127% |
| Urine | 108% | 90%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 531.9 | 18.2 | 3.4 |
| 2 | 20 | 2,019.4 | 63.1 | 3.1 |
| 3 | 20 | 7,679.1 | 273.4 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 583.4 | 37.5 | 6.4 |
| 2 | 24 | 2,306.4 | 182.9 | 7.9 |
| 3 | 24 | 7,538.8 | 608.2 | 8.1 |
Background Information
Epidermal growth factor (EGF) is a member of the epidermal growth factor superfamily. EGF preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF plays important roles in multiple biological processes, such as the regulation of cell growth, proliferation, and differentiation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EGF, HOMG4, Pro epidermal growth factor, URG, Urogastrone |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Aging Cell Activation of epidermal growth factor receptor signaling mediates cellular senescence induced by certain pro-inflammatory cytokines. | ||
Exp Eye Res High-throughput transcriptional profiling combined with angiogenesis antibody array analysis in an orbital venous malformation cohort. | ||
Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma | ||
J Neurotrauma Regulation of the Maturity of Dental Pulp-Derived Induced Neural Cells by Cell-Recognizing Cadherin-Fc Chimeric Antibody Improved Neurological Function after Cell Implantation in a Mouse Traumatic Brain Injury Model. | ||
Indian J Hematol Blood Transfus Experimental Study on the Influence of Different Storage Conditions (Temperature, Duration) on Platelet Count, LDH, and Growth Factor Concentration in Platelet-Rich Plasma. |
