Human E-selectin /CD62E ELISA Kit
Sensitivity
0.05 ng/mL
Range
0.5-16 ng/mL
Reactivity
Human
Cat no : KE00169
Synonyms
CD62E, E-selectin, CD62 antigen-like family member E, E selectin, ELAM
Validation Data Gallery
Product Information
KE00169 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The E-Selectin ELISA kit is to be used to detect and quantify protein levels of endogenous E-Selectin. The assay recognizes human E-Selectin. An antibody specific for E-Selectin has been pre-coated onto the microwells. The E-Selectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for E-Selectin is added to detect the captured E-Selectin protein.For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human E-selectin /CD62E ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.5-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6401 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 100% | 83%-117% |
| Human plasma | 89% | 72%-104% |
| cell culture supernatants | 95% | 74%-123% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 0.47 | 0.02 | 4.63 |
| 2 | 20 | 2.12 | 0.05 | 2.43 |
| 3 | 20 | 8.32 | 0.15 | 1.85 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 0.55 | 0.06 | 10.37 |
| 2 | 24 | 2.05 | 0.11 | 5.21 |
| 3 | 24 | 8.22 | 0.37 | 4.48 |
Background Information
E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also include L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD62E, E-selectin, CD62 antigen-like family member E, E selectin, ELAM |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
