AuthentiKine™ Human Cystatin C ELISA Kit
Cat no : KE00150
Synonyms
ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin
Validation Data Gallery
Product Information
KE00150 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Cystatin C ELISA kit is to be used to detect and quantify protein levels of endogenous Cystatin C. The assay recognizes human Cystatin C. An antibody specific for Cystatin C has been pre-coated onto the microwells. The Cystatin C protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Cystatin C is added to detect the captured Cystatin C protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human Cystatin C ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants, Urine, Saliva, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 10.5 pg/mL |
| Range | 15.6 -500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1471 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89% | 76%-95% |
| Cell culture supernatants | 86% | 73%-94% |
| Urine | 97% | 81%-107% |
| Saliva | 89% | 81%-98% |
| Human milk | 94% | 87%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 108.1 | 8.3 | 7.6 |
| 2 | 20 | 203.8 | 16.4 | 8.0 |
| 3 | 20 | 348.3 | 23.5 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 100.3 | 6.2 | 6.2 |
| 2 | 24 | 211.9 | 18.7 | 8.8 |
| 3 | 24 | 370.6 | 26.2 | 7.1 |
Background Information
Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Crit Care A translational study of Galectin-3 as an early biomarker and potential therapeutic target for ischemic-reperfusion induced acute kidney injury. | ||
Eur Geriatr Med Blood and urine cell-free circulating DNA in older adults with sarcopenia: a hypothesis-generating study. |
