Human Cyclin E1 ELISA Kit
Sensitivity
0.04 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Cat no : KE00393
Synonyms
CCNE1, CCNE, Cyclin E, G1/S specific cyclin E1, G1/S-specific cyclin-E1
Validation Data Gallery
Product Information
KE00393 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Cyclin E1 ELISA kit is to be used to detect and quantify protein levels of endogenous human Cyclin E1. The assay recognizes human Cyclin E1. An antibody specific for human Cyclin E1 has been pre-coated onto the microwells. The human Cyclin E1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Cyclin E1 is added to detect the captured human Cyclin E1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Cyclin E1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 898 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 93%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 8.67 | 0.40 | 4.60 |
| 2 | 20 | 2.07 | 0.08 | 3.95 |
| 3 | 20 | 0.51 | 0.04 | 7.58 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.40 | 0.32 | 3.40 |
| 2 | 24 | 2.29 | 0.09 | 3.78 |
| 3 | 24 | 0.60 | 0.04 | 6.32 |
Background Information
Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating cyclin-dependent kinase 2 (CDK2), regulates the G1-S phase transition of the mammalian cell division cycle.The transition from the quiescent state of the cell cycle into the phase of DNA synthesis is thought to be regulated by E-type cyclins Cyclin E1 (CCNE1) and Cyclin E2 (CCNE2) in complex with CDK2. During G1/S phase, cyclin dependent kinases (CDKs) (CDK4/6) is activated by cyclin D1, D2, or D3 to phosphorylate Rb, followed by phosphorylation by CDK2 in complex with cyclin E1 or cyclin E2. Cyclin E1 is highly related to cyclin E2, and both cyclin E1 and cyclin E2 are estrogen target genes that can facilitate anti-estrogen resistance and can be highly expressed in breast cancer. Amplified expressed cyclin E1 is a well-known tumorigenic factor and prognostic biomarker in several malignancies, such as osteosarcoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCNE1, CCNE, Cyclin E, G1/S specific cyclin E1, G1/S-specific cyclin-E1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
