Human Cyclin D1 ELISA Kit
Sensitivity
0.16 ng/mL
Range
0.25-16 ng/mL
Reactivity
Human
Cat no : KE00344
Synonyms
B cell lymphoma 1 protein, CCND1, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31
Validation Data Gallery
Product Information
KE00344 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Cyclin D1 ELISA kit is to be used to detect and quantify protein levels of endogenous human Cyclin D1. The assay recognizes human Cyclin D1. An antibody specific for human Cyclin D1 has been pre-coated onto the microwells. The human Cyclin D1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Cyclin D1 is added to detect the captured human Cyclin D1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Cyclin D1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.16 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 595 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 79%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 7.51 | 0.27 | 3.55 |
| 2 | 20 | 2.02 | 0.10 | 4.81 |
| 3 | 20 | 0.59 | 0.05 | 8.95 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.28 | 0.84 | 9.02 |
| 2 | 24 | 2.20 | 0.13 | 6.11 |
| 3 | 24 | 0.51 | 0.04 | 8.21 |
Background Information
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 is mostly known for the role played in the nucleus, as regulator of cell cycle progression. Cyclin D1 modulates the transition from G1 to S phase through its action as allosteric regulator of the cyclin-dependent kinase 4 (CDK) and CDK6 (PMID: 33317149). The active Cyclin D1/CDK4 complex translocates into the nucleus, phosphorylates retinoblastoma (RB) protein together with Cyclin E/CDK2, and dismisses the repressive action on the E2F transcription factor, which regulates the transcription of specific genes required for cell proliferation (PMID: 8449399, PMID: 9447971). Dysregulation of Cyclin D1 transcription, accumulation and ubiquitination, as well as assembly and hyperactivation of its cognate CDK, lead to uncontrolled cell growth. Thus, Cyclin D1 is regarded as an oncogenic driver in different types of cancers including breast cancer, lung cancer, and melanoma (PMID: 31885322). Cyclin D1 is expressed by the majority of normal human cells apart from cells derived from bone marrow stem cell lines (PMID: 1386335).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B cell lymphoma 1 protein, CCND1, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
