Human Complement factor D ELISA Kit
Sensitivity
1.3 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Cat no : KE00836
Synonyms
CFD, ADIPSIN, C3 convertase activator, complement factor D (adipsin), DF
Validation Data Gallery
Product Information
KE00836 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Complement factor D ELISA kit is to be used to detect and quantify protein levels of endogenous human Complement factor D. The assay recognizes human Complement factor D. An antibody specific for human Complement factor D has been pre-coated onto the microwells. The human Complement factor D protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Complement factor D is added to detect the captured human Complement factor D protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Complement factor D ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1675 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 95%-104% |
| Urine | 98% | 95%-102% |
| Cell culture supernatant | 96% | 92%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 259.3 | 5.5 | 2.1 |
| 2 | 8 | 61.0 | 2.0 | 3.3 |
| 3 | 8 | 32.8 | 0.8 | 2.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 257.9 | 5.0 | 1.9 |
| 2 | 16 | 61.0 | 1.7 | 2.7 |
| 3 | 16 | 31.9 | 1.4 | 4.4 |
Background Information
Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CFD, ADIPSIN, C3 convertase activator, complement factor D (adipsin), DF |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
