Human Collagen Type I ELISA Kit
Cat no : KE00395
Synonyms
COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain
Validation Data Gallery
Product Information
KE00395 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Collagen Type I ELISA kit is to be used to detect and quantify protein levels of endogenous human Collagen Type I. The assay recognizes human Collagen Type I. An antibody specific for human Collagen Type I has been pre-coated onto the microwells. The human Collagen Type I protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Collagen Type I is added to detect the captured human Collagen Type I protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Collagen Type I ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1278 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 86%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.03 | 0.10 | 1.96 |
| 2 | 20 | 1.75 | 0.06 | 3.34 |
| 3 | 20 | 0.51 | 0.03 | 5.40 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.14 | 0.35 | 6.71 |
| 2 | 24 | 1.53 | 0.14 | 9.44 |
| 3 | 24 | 0.68 | 0.06 | 9.27 |
Background Information
Type I collagen is the main structural component of connective tissues such as skin, tendon and bone, and it is the most abundant and widely expressed collagen in human body. Collagen type I is a heterotrimer, which contains one α2(I) and two α1(I) chains, which are encoded by unlinked loci COL1A2 and COL1A1, respectively. COL1A2 gene mutation is associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis and atypical Marfan syndrome. This product mainly recognizes the C-terminal propeptide.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology Platelet-hitchhike nanomedicine for theranostics of coronary artery dissection via X-CT imaging and inhibiting NLRP3 signaling pathway. | ||
NPJ Sci Food From rosemary and coffee to bioactive nanovesicles: exploring new frontiers in food functional ingredients. |
