Recombinant Human Factor IX/PTC protein (His Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
His Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human Factor IX protein Thr29-Thr461 (Accession# P00740-1) with a His tag at the C-terminus. |
| 基因ID | 2158 |
| 蛋白编号 | P00740-1 |
| 预测分子量 | 49.8 kDa |
| SDS-PAGE | 55-70 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Factor IX, also known as PTC, circulates as an inactive zymogen until the proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Upon activation by Factor XIa or Factor VIIa/tissue factor complex, Factor IXa forms a complex with Factor VIIIa, calcium ions, and phospholipids on the surface of activated platelets. This complex, known as the tenase complex, efficiently activates Factor X to Factor Xa, leading to thrombin generation and fibrin clot formation. Deficiencies or mutations in Factor IX result in Hemophilia B, an X-linked recessive bleeding disorder.
参考文献:
1. Davie, E W et al. Biochemistry vol. 30,43 (1991): 10363-70. 2. Ahmad, S S et al. Journal of thrombosis and haemostasis : JTH vol. 1,11 (2003): 2348-55. 3. Girolami, Antonio et al. Seminars in thrombosis and hemostasis vol. 41,4 (2015): 359-65.
